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Clinical evidence records

GHK-Cu: clinical evidence record

Also known as: Copper Tripeptide-1, Glycyl-L-Histidyl-L-Lysine Copper, Prezatide

Evidence: Mixed evidence
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Oliver Mackman · Editorial director · Best Business Loans Ltd (16833937)

Last updated 2026-05-22

Editorial with affiliate links. We earn from purchases via outbound retailer / clinic links. How we are funded.

AI-friendly summary · GHK-Cu

Discovered by Loren Pickart in 1973. Endogenous in human plasma, declining with age. Extensive cosmetic and wound-healing literature, with limited human RCT-grade efficacy work outside topical formulations. Injectable form is research-tier.

Mechanism of action

How GHK-Cu works

Naturally occurring tripeptide (Glycyl-L-Histidyl-L-Lysine) that complexes copper ions with high affinity. Proposed to modulate gene expression linked to extracellular matrix synthesis, antioxidant signalling, and wound-healing pathways in preclinical and in-vitro models.

Top peer-reviewed citations

Selection of the most-cited peer-reviewed literature on GHK-Cu. Where a verified PMID or DOI is shown, the citation links to the original record. Other citations list the title, authors, journal and year so the reader can locate the paper through the journal index or the PubMed search linked below. PeptideClear publishes editorial commentary, not clinical guidance.

  1. GHK Peptide as a Natural Modulator of Multiple Cellular Pathways in Skin Regeneration

    Pickart L, Vasquez-Soltero JM, Margolina A. BioMed Research International, 2015.

    PMID: 26236730 DOI: 10.1155/2015/648108

  2. The human tripeptide GHK-Cu in prevention of oxidative stress

    Pickart L, Vasquez-Soltero JM, Margolina A. Oxidative Medicine and Cellular Longevity, 2012.

    PMID: 22666519 DOI: 10.1155/2012/324832

  3. Tripeptide in human serum which prolongs survival of normal liver cells and stimulates growth in neoplastic liver

    Pickart L, Thaler MM. Nature New Biology, 1973.

    PMID: 4349963

  4. GHK and DNA: resetting the human genome to health

    Pickart L, Vasquez-Soltero JM, Margolina A. BioMed Research International, 2014.

    PMID: 25302294 DOI: 10.1155/2014/151479

  5. Tripeptide-copper complex stimulation of dermal fibroblast collagen synthesis

    Maquart FX, Pickart L, Laurent M, et al.. FEBS Letters, 1988.

    PMID: 3169264 DOI: 10.1016/0014-5793(88)80509-x

  6. Regenerative and Protective Actions of the GHK-Cu Peptide in the Light of the New Gene Data

    Pickart L, Margolina A. International journal of molecular sciences, 2018.

    PMID: 29986520 DOI: 10.3390/ijms19071987

  7. Protective effects of GHK-Cu in bleomycin-induced pulmonary fibrosis via anti-oxidative stress and anti-inflammation pathways

    Ma WH, Li M, Ma HF, et al.. Life sciences, 2020.

    PMID: 31809714 DOI: 10.1016/j.lfs.2019.117139

  8. The tri-peptide GHK-Cu complex ameliorates lipopolysaccharide-induced acute lung injury in mice

    Park JR, Lee H, Kim SI, et al.. Oncotarget, 2016.

    PMID: 27517151 DOI: 10.18632/oncotarget.11168

  9. Effect of Gly-Gly-His, Gly-His-Lys and their copper complexes on TNF-alpha-dependent IL-6 secretion in normal human dermal fibroblasts

    Gruchlik A, Jurzak M, Chodurek E, et al.. Acta poloniae pharmaceutica, 2012.

    PMID: 23285694

  10. Are We Ready to Measure Skin Permeation of Modern Antiaging GHK-Cu Tripeptide Encapsulated in Liposomes?

    Ogórek K, Nowak K, Wadych E, et al.. Molecules (Basel, Switzerland), 2025.

    PMID: 39795193 DOI: 10.3390/molecules30010136

Full PubMed search: https://pubmed.ncbi.nlm.nih.gov/?term=GHK-Cu+copper+tripeptide.

UK regulatory status

Plain-English summary of where GHK-Cu sits under the four UK and international frameworks that govern peptide supply. Editorial commentary, not legal advice.

  • Misuse of Drugs Act 1971: Not controlled under the Misuse of Drugs Act 1971.
  • Psychoactive Substances Act 2016: Not scheduled under the Psychoactive Substances Act 2016.
  • MHRA medicines classification: No UK marketing authorisation as a medicine.
  • WADA Prohibited List: Not currently listed on the WADA Prohibited List.

Full regulatory record: GHK-Cu UK regulatory status.

Risks and unknowns

What the literature does not yet show about GHK-Cu

Known concerns

Open questions in the literature

Regulatory note

No UK marketing authorisation as a medicine. Not currently listed on the WADA Prohibited List. The moment a UK seller or commentator makes a therapeutic claim, MHRA can treat the product as an unlicensed medicinal product.

Important: PeptideClear publishes encyclopedia commentary only and does not recommend human use. Speak to a UK-registered prescriber before any medical decision.

Related reading on PeptideClear

Frequently asked questions

What is the evidence level for GHK-Cu?
Discovered by Loren Pickart in 1973. Endogenous in human plasma, declining with age. Extensive cosmetic and wound-healing literature, with limited human RCT-grade efficacy work outside topical formulations. Injectable form is research-tier.
What is the UK regulatory status of GHK-Cu?
No UK marketing authorisation as a medicine. Not controlled under the Misuse of Drugs Act 1971. Not scheduled under the Psychoactive Substances Act 2016. Not currently listed on the WADA Prohibited List.
Has GHK-Cu been tested in human clinical trials?
GHK-Cu has some early-phase human work alongside a larger preclinical animal-model literature. No phase III randomised clinical trial has produced a UK marketing authorisation. See the cited peer-reviewed literature above.
Where can I read the source literature for GHK-Cu?
The full PubMed search is linked above. Each cited paper has its title, author list and journal. Where we have a verified PMID or DOI the citation links directly. Other citations can be located by the reader through the journal index or the PubMed search.

Last verified 2026-05-22. Editorial commentary, not legal or clinical advice. Citations without a linked identifier can be located through the PubMed search and the journal index.

Reviewed by Oliver Mackman, editorial director · last reviewed 2026-05-22T12:00:00.000Z